Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Baz1b

Cell type

Cell type Class
Neural
Cell type
Nucleus Accumbens
MeSH Description
Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA.

Attributes by original data submitter

Sample

source_name
nucleus accumbens
strain
C57BL/6J
treatment
Resilient
antibody
WSTF, abcam ab51256

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For each ChIP-sequencing (ChIP-seq) replicate, bilateral 14 Ga NAc punches were pooled from five to 10 mice. Tissue was lightly fixed to cross-link DNA with associated proteins and the material was further sheared using a Bioruptor to obtain mostly 100–300 bp fragments and immunoprecipitated. Immunoprecipitated DNA were prepared for ChIP-seq using an Illumina kit according to the manufacturer’s instructions. Each experimental condition was analyzed with independent biological triplicates. Amplification and size selection were confirmed with a BioAnalyzer. The resulting libraries were sequenced on an Illumina HiSeq 2500 with 100 bp read length.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
58087695
Reads aligned (%)
99.8
Duplicates removed (%)
60.3
Number of peaks
163 (qval < 1E-05)

mm9

Number of total reads
58087695
Reads aligned (%)
99.8
Duplicates removed (%)
60.3
Number of peaks
158 (qval < 1E-05)

Base call quality data from DBCLS SRA