Antigen

Antigen Class

TFs and others

Antigen

Baz1b

Cell type

Cell type Class

Neural

Cell type

Nucleus Accumbens

MeSH Description

Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA.

Sample

source_name

nucleus accumbens

strain

C57BL/6J

treatment

Resilient

antibody

WSTF, abcam ab51256

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For each ChIP-sequencing (ChIP-seq) replicate, bilateral 14 Ga NAc punches were pooled from five to 10 mice. Tissue was lightly fixed to cross-link DNA with associated proteins and the material was further sheared using a Bioruptor to obtain mostly 100–300 bp fragments and immunoprecipitated. Immunoprecipitated DNA were prepared for ChIP-seq using an Illumina kit according to the manufacturer’s instructions. Each experimental condition was analyzed with independent biological triplicates. Amplification and size selection were confirmed with a BioAnalyzer. The resulting libraries were sequenced on an Illumina HiSeq 2500 with 100 bp read length.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

58087695

Reads aligned (%)

99.8

Duplicates removed (%)

60.3

Number of peaks

163 (qval < 1E-05)

mm9

Number of total reads

58087695

Reads aligned (%)

99.8

Duplicates removed (%)

60.3

Number of peaks

158 (qval < 1E-05)